FastQCFastQC Report
Sun 19 Mar 2023
SRR4895796_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895796_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886327
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT107881.2171580015050878No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT15380.17352512108962043No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT13960.15750394606053972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATA100.007206885144.214059
TGAGCCA887450.0144.019042
CTGAGCC887550.0144.002821
GAGCCAT321050.0143.958863
GAGCCAG290550.0143.436613
AGCCAGT99700.0143.338074
AGCCATA90550.0143.330024
GAGCCAA261450.0143.323433
AGCCATG132450.0143.226034
GCCATAG31600.0142.380525
GCCAGTA31300.0142.132665
AGCCAGA83600.0141.877234
AGCCATT97300.0141.538184
AGCCAAG91550.0141.291874
GCCATTG34600.0141.288455
GCCATAT29250.0141.001335
AGCCAGG106650.0140.825564
GCCAGAT27200.0140.759815
AGCCAAT93050.0140.641434
GCCATGA31750.0140.345285
Produced by FastQC (version 0.11.5)