FastQCFastQC Report
Sun 19 Mar 2023
SRR4895796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886327
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG240632.71491221637161No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66930.7551389047157538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA39700.4479159497566925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG38400.4332486768427454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC34080.38450820069793656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA25390.28646312252701317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22510.25396947176380724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG20120.22700425463739682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC17310.19530038010801884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGTACTGTGGCTCA16280.18367938695312225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACA11910.13437478492700777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG9450.10661979156676939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT387550.0109.487261
AGCAGTG398150.0106.384252
GGTATCA409950.0103.192478
CAGTGGT409550.0103.033534
GTGGTAT409350.0102.983686
TGGTATC410100.0102.9249047
GTATCAA412000.0102.643819
AGTGGTA414550.0101.662645
GCAGTGG422200.099.929253
CGGAGCC3700.039.18919145
CGGTCTG3100.032.741936145
AGCGGTA1652.3860248E-630.7575765
CCGGCTT2202.2635504E-829.65909145
AAATATA24550.028.350304145
CGGTATC1804.69917E-628.1944457
CTCGGCG3005.857146E-1026.583332145
GTATCAC3607.2759576E-1226.1805559
ATGGGGG42200.026.08026525-29
CCGTCTT7000.025.892859145
CATGGGG87800.025.56578625-29
Produced by FastQC (version 0.11.5)