FastQCFastQC Report
Sun 19 Mar 2023
SRR4895795_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895795_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054950
Sequences flagged as poor quality0
Sequence length151
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG1056250.0144.01091
CAATGAG349400.0143.85823
TCAATGA1057450.0143.847462
CAATGAT372050.0143.413253
CAATGAA324800.0142.723373
CAATGAC15250.0139.933613
AATGAGT126500.0139.515064
AATGAGG124800.0139.451394
AATGATG137750.0139.006994
AATGAAG115500.0138.508214
AATGAGA105850.0136.764394
AATGATA119600.0135.749244
AATGAAT121050.0135.552554
ATGAGTA41450.0132.882875
ATGATAG41550.0132.389545
AATGATT133300.0131.857164
ATGAGTG41500.0131.854175
ATGAAGT41150.0131.749275
ATGATGA42350.0130.739845
ATGAGGA37300.0130.658625
Produced by FastQC (version 0.11.5)