FastQCFastQC Report
Sun 19 Mar 2023
SRR4895795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054950
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG159651.5133418645433434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132001.251244134793118No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG129081.223565097871937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC100520.9528413668894261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21490.20370633679321296No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA20350.19290013744727239No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA16530.15668989051613819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA14250.13507749182425707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT14130.13393999715626334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT445000.0117.881881
GGTATCA451300.0116.305148
GTATCAA453100.0115.885619
GTGGTAT451350.0115.762196
TGGTATC452500.0115.580147
AGCAGTG455350.0115.101452
CAGTGGT454300.0115.096144
GCAGTGG457150.0114.251713
AGTGGTA461450.0113.2656255
TAGCGAC604.9412774E-448.3411252
AAATATA41900.043.602577145
CGGTCTG2150.040.463963145
CTCAATG7950.038.3098751
AGGGGTA3250.035.6980635
GCGGTAT900.003628825832.2213066
TCAATGA10150.029.2904362
ATAGCGA1000.00606841829.0060521
GGGTATC3900.027.8838217
AGCGGTA1050.007702580727.6234975
GAAACCG27300.026.8734285-89
Produced by FastQC (version 0.11.5)