FastQCFastQC Report
Sun 19 Mar 2023
SRR4895794_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895794_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463237
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGATCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4690.10124407160913312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGA202.1908654E-6144.173146
GCTGATC463550.0144.064292
CGCTGAT463650.0144.033221
CTGATCG156300.0143.942553
CTGATCA137650.0143.701813
TGATCAG48850.0143.582874
CTGATCT154250.0143.565613
TGATCGA46200.0143.392994
GATCATG18950.0143.031945
TGATCGT59750.0142.846024
GATCTAG18250.0142.593155
TGATCAT47100.0142.48964
TGATCGG49250.0142.416734
TGATCTG61500.0142.414934
GATCAGT16100.0141.934435
GATCGGT18200.0141.796665
TGATCAA41300.0141.729524
GATCGAG17150.0141.651175
GATCAAG15000.0141.289675
GATCTGG21250.0140.780845
Produced by FastQC (version 0.11.5)