FastQCFastQC Report
Sun 19 Mar 2023
SRR4895794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463237
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG98682.1302270759891804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62521.349633125160555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47451.024313688241656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18810.4060556475411075No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA14350.3097766370130193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8080.17442475449931674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5960.12865984366533761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT151800.0100.673571
GTGGTAT153300.099.047836
GGTATCA154600.098.7777948
GTATCAA155000.098.68879
AGCAGTG155000.098.429312
CAGTGGT155000.098.148624
TGGTATC155750.097.5828867
GCAGTGG157950.096.3614353
AGTGGTA160550.094.665435
GAGGTAT1453.8380676E-1045.0075046
AAATATA16900.043.755245145
CGCTGAT1859.640644E-1139.1872631
AGGTATC1502.5906047E-838.6731157
CGGTCTG1102.434843E-432.952927145
AGAGGTA2008.914867E-932.6304445
AGCGAGC900.003627108932.22064145
CCGCAAG3600.030.206848145
TGCGGCT1804.694608E-628.193058145
GCAGTAG1358.068261E-426.856333
CGACCTA1200.025.37375345-49
Produced by FastQC (version 0.11.5)