FastQCFastQC Report
Sun 19 Mar 2023
SRR4895793_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895793_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458272
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT92162.011032749109699No Hit
CGGATTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG6130.13376335451434956No Hit
CGGATTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATACCACT5310.11587005097409399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTGC458950.0143.975712
CGGATTG459050.0143.944351
GATTGCG171000.0143.858343
GATTGCT151050.0143.718323
ATTGCGA42600.0143.68794
GATTGCA122750.0143.432083
ATTGCAG41300.0143.148214
ATTGCTG53150.0143.110444
ATTGCGT72700.0143.104754
ATTGCGG54150.0142.198474
ATTGCTA42800.0141.83734
ATTGCAT43300.0140.199464
TTGCGAT15500.0140.009315
GATTGCC15450.0139.995773
TTGCGGA14850.0138.855065
TTGCGTA16100.0138.821885
TTGCTGA13900.0138.490055
TTGCGAG13850.0138.469455
TGCGAGT4950.0138.369556
ATTGCAA38800.0138.063614
Produced by FastQC (version 0.11.5)