FastQCFastQC Report
Sun 19 Mar 2023
SRR4895793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458272
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG237005.171601145171426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA41370.9027389847077717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25340.5529467215976538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA9590.2092643670134767No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5940.12961734515746107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT236000.069.016161
AGTGGTA238050.068.1402055
GTATCAA239150.067.721059
GTGGTAT239800.067.484386
TGGTATC240500.067.318117
GGTATCA240850.067.310598
CAGTGGT243000.066.886134
AGCAGTG244600.066.626522
GCAGTGG246450.066.008663
CGGTCTG1258.913048E-1152.196743145
CGTGCGA1050.007706512727.617325145
CGCAAGG2001.0643427E-525.373415145
AGTCGGA1500.001492634424.1677956
CGACCTA1200.024.16568445-49
CGTGGGG2401.5957994E-624.165157145
TGCGGCT1801.5191764E-424.165157145
CGTTCGG452.6547548E-522.55414655-59
TACGCAC1300.022.30629750-54
CATGGGT15250.022.06100825-29
CTCGCGC403.103751E-421.75101540-44
Produced by FastQC (version 0.11.5)