FastQCFastQC Report
Sun 19 Mar 2023
SRR4895792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378421
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG97892.5868014724341406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23930.6323644829435999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15870.4193741890645604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8400.2219749961022248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT96200.074.914031
AGTGGTA97200.074.21795
GGTATCA98300.073.5251858
GTATCAA98400.073.376799
AGCAGTG99000.072.941722
GTGGTAT98900.072.859236
CAGTGGT99400.072.575254
TGGTATC99500.072.4198767
GCAGTGG102750.070.209053
CGACACA858.9079913E-751.17811
CGAATTT450.0096383648.3284879
GACACAC1251.2627384E-534.801112
CGGTCTG900.003627184532.21899145
CGCTGTT1555.4952718E-528.061705145
CGACCTA1150.027.7363545-49
TTAGCGC502.2829954E-623.19767440-44
CATGGGG19800.022.48482325-29
CGGCGGT1550.022.4541135-39
TCTCGCA1500.022.23521870-74
TACGCAC1500.022.23110650-54
Produced by FastQC (version 0.11.5)