FastQCFastQC Report
Sun 19 Mar 2023
SRR4895791_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895791_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794671
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC796750.0143.828552
TCTATCG267200.0143.507033
CCTCTAT798700.0143.47741
CTATCGA73600.0143.1314
CTATCAG79200.0143.116154
CTATCGT98250.0143.107824
CTATCGG93400.0142.819064
TCTATCA232650.0142.782993
TATCGAT26600.0142.582245
TCTATCT272950.0142.438723
CTATCTG94850.0142.384174
CTATCTA79400.0142.029164
TATCGTG32800.0141.790515
TATCGAG22700.0141.349885
TATCGGT32750.0141.346485
CTATCAT84800.0141.317674
CTATCAA68100.0140.926364
TATCGTA28300.0140.896445
TATCATG33200.0139.647835
TATCGAA21350.0139.48055
Produced by FastQC (version 0.11.5)