FastQCFastQC Report
Sun 19 Mar 2023
SRR4895791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794671
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG151431.9055684679571798No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57470.7231923651423042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG27110.3411474685750455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23390.2943356433039585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15350.193161698363222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8440.1062074745397781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT187750.084.432021
GGTATCA191700.082.671478
AGTGGTA191450.082.552225
GTGGTAT191450.082.476496
CAGTGGT192200.082.2078864
GTATCAA193500.081.972219
TGGTATC193000.081.851687
AGCAGTG195050.081.266932
GCAGTGG198000.079.8363953
TCTATCG1351.024091E-842.970053
CGGTCTG2100.041.424976145
CTCTATC3650.027.8128052
CCTCTAT4000.027.1936951
CCGCAAG4700.026.221128145
TGCGGCT3452.9649527E-923.113935145
CATGGGG41150.022.72899825-29
CGACCTA2200.022.40742945-49
CTATCGC555.219319E-621.089876140-144
TACGCAC2300.020.80358950-54
GCCCCAA6650.020.712486145
Produced by FastQC (version 0.11.5)