FastQCFastQC Report
Sun 19 Mar 2023
SRR4895790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564987
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG560059.912617458454797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA85721.5172030506896619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31910.5647917562704983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25020.44284204769313273No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA14150.25044824040199154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC11580.20496046811696556No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10560.18690695538127425No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7920.1401802165359557No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6520.11540088533010494No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6310.11168398564922732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGATTAGGAGATCGGAAGAGCAC6080.10761309552255185No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG5950.1053121576248657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT443550.066.7811361
AGCAGTG459550.064.428852
AGTGGTA461200.064.198345
GGTATCA467300.063.732668
GTATCAA468150.063.678899
GTGGTAT467800.063.3545846
CAGTGGT468150.063.2762534
TGGTATC468650.063.255157
GCAGTGG473650.062.449653
TAGATCG450.00963454148.336433145
CGGTCTG907.475476E-540.28036145
CGTCGGC900.003625766832.22429145
GCCGGCT4250.030.707851145
CGTGGGC4100.028.294497145
CACGGCT1855.807371E-627.434189145
CACGGGC1358.075314E-426.85357145
TGCGGGC1450.001225324625.001604145
CACGGGT2051.2883314E-524.757685145
GGGTATC2951.425542E-824.5756727
GGGGTAT2951.425542E-824.5756726
Produced by FastQC (version 0.11.5)