FastQCFastQC Report
Sun 19 Mar 2023
SRR4895789_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895789_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727990
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACAACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9670.13283149493811727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC730300.0143.739972
CCGACAA730750.0143.651461
GACAACT248500.0143.507643
GACAACG254850.0142.959153
ACAACGT92450.0142.410224
GACAACA209050.0141.651723
ACAACTG90800.0141.583564
CAACTGT30050.0141.324725
CAACGGT30900.0139.53725
CAACTGA24350.0139.46635
ACAACGG90700.0139.195824
CAACGTA27350.0139.195085
CAACGTG31550.0139.176285
CAACTTG30400.0138.511735
CAACTAG24650.0138.061435
CAACGTT29950.0137.703985
ACAACTA74900.0137.369234
CAACGAT26100.0137.021425
ACAACGA73950.0136.988924
ACAACTT88300.0136.936634
Produced by FastQC (version 0.11.5)