FastQCFastQC Report
Sun 19 Mar 2023
SRR4895789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727990
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG123641.6983749776782648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83431.1460322257173863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20710.2844819296968365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT8620.11840821989313041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC8570.11772139727194056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8530.11717193917498868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT163600.089.743751
AGTGGTA163200.089.602145
GGTATCA166500.088.131058
GTGGTAT166050.087.933276
AGCAGTG167200.087.594652
CAGTGGT167250.087.4384164
TGGTATC167800.087.145837
GTATCAA169300.086.710349
GCAGTGG170550.085.619023
CGGTCTG2450.038.468914145
GCCCCAA8100.026.851522145
TACGCAC3150.025.31575850-54
GGTCTGA4500.024.166368145
CGACCTA3350.023.37124345-49
CATGGGG40950.023.22963725-29
TGCGGCT5050.022.970013145
AGCCGTA1600.002175609822.6544159
TCTCGCA3750.021.26903570-74
ATAGGCG1600.019.937254100-104
GGCGATA1950.019.334425105-109
Produced by FastQC (version 0.11.5)