FastQCFastQC Report
Sun 19 Mar 2023
SRR4895788_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895788_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411987
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC152903.7112821521067416No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG77421.8791855082806013No Hit
CCATCCTCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC25700.6238060909688898No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATACC12440.3019512751615949No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGCACTCTGCGTTGATACC8550.20753082014723767No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATACC8150.19782177592982303No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG8140.19757904982438765No Hit
CCATCCTCTGGGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC7010.17015099991019134No Hit
CCATCCTCTGCGTTCATACCACTGCTTCCCATGTACTCTGCGTTGATACC5540.1344702624111926No Hit
CCATCCTCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC5170.12548939651008406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAG100.0072027314144.232548
CATCCTC413700.0143.622422
CCATCCT414050.0143.501021
ATCCTCG127550.0143.4413
ATCCTCA117150.0143.12453
ATCCTCT158400.0142.456973
TCCTCGA33100.0141.835944
CCTCGTA14600.0141.762825
CTCGAGT3950.0140.581096
CTCATGT22000.0140.298936
CCTCATG29950.0140.139145
TCCTCTG85750.0140.111634
TCCTCAT53800.0140.077154
TCCTCAG34350.0140.033654
CTCGTAG6650.0138.810296
CCTCGAT11650.0138.661335
TCGAAGT1150.0137.961587
CTCGAAT3400.0137.869356
TCCTCGG48700.0137.716924
TCATGTA19200.0137.471657
Produced by FastQC (version 0.11.5)