FastQCFastQC Report
Sun 19 Mar 2023
SRR4895787_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895787_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707122
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9500.1343473969131211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC708450.0143.907822
CACCTTA709850.0143.624011
CTTACTG81200.0143.561114
CCTTACG233300.0143.471883
CTTACGT86050.0143.009674
CTTACGA63000.0142.580554
CCTTACA205850.0142.536583
CTTACAG64900.0142.405294
CCTTACT251000.0142.373113
CTTACGG83500.0142.36954
CTTACTA73800.0140.958984
TTACGAG19300.0140.82085
TTACGTG26300.0140.07095
TTACGTA25950.0139.737645
CTTACAT78950.0139.616964
TTACGAT24350.0139.445575
TTACGGT31800.0139.195115
TTACGGA22150.0138.975085
CTTACAA63250.0138.939584
CTTACTT95800.0137.861424
Produced by FastQC (version 0.11.5)