FastQCFastQC Report
Sun 19 Mar 2023
SRR4895787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707122
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG110081.556732784441723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99521.4073950463993485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16980.24012829469313637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA155150.093.4137655
GGTATCA158350.091.983898
AAGCAGT158500.091.811841
GTATCAA158900.091.795899
GTGGTAT158950.091.089316
TGGTATC159550.090.701327
AGCAGTG160600.090.521022
CAGTGGT160900.090.171984
GCAGTGG162400.089.249823
AGCGGTA752.5494075E-548.3346525
CGGTCTG1351.0257281E-842.9611145
ACCTTAC3450.029.4231722
CGGTATC1255.151508E-429.0007927
CGATCGT1150.025.219505120-124
CACCTTA4103.8198777E-1122.9900591
GCGGTAT1600.002174178422.656876
TTCGATA1600.002175072222.655268145
CATGGGG34400.022.2982625-29
GAGGTAT2053.6642986E-421.220096
GTCGATC1400.020.716022115-119
Produced by FastQC (version 0.11.5)