FastQCFastQC Report
Sun 19 Mar 2023
SRR4895786_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895786_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584271
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGAGC586350.0143.69082
CAAGGAG587150.0143.495031
AGGAGCT203050.0143.461563
GGAGCTA59350.0143.356864
AGGAGCA166850.0143.17013
AGGAGCG200250.0142.694993
GAGCTTA22050.0142.245275
GGAGCGT74800.0142.182974
GGAGCAT58500.0141.865434
GAGCTTG25650.0141.677325
GAGCTAT19500.0141.618945
GAGCTAG19000.0141.550835
GAGCGAT21100.0141.473485
GAGCTAA19500.0141.249185
GGAGCTT77150.0141.21664
GAGCGTA23050.0141.079135
GGAGCTG66750.0141.074684
GAGCGTT24600.0140.983125
GGAGCGA58450.0140.75324
GAGCTGA16150.0140.18915
Produced by FastQC (version 0.11.5)