FastQCFastQC Report
Sun 19 Mar 2023
SRR4895786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584271
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG75521.292550888200852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35960.6154678222947912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14010.2397859897205235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11770.20144761591795587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6680.11433050758979993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5900.10098053814069158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT118650.094.342991
GTGGTAT121550.091.9649356
GGTATCA123050.091.4408348
GTATCAA122900.091.3754659
AGTGGTA122700.091.051715
TGGTATC123400.090.535217
AGCAGTG123600.090.506032
CAGTGGT123900.090.169864
GCAGTGG128450.086.975833
AGGTATC1401.412991E-841.427817
GAGGTAT1907.1545437E-626.707756
CGACCTA1600.026.2789745-49
AGAGGTA2001.064631E-525.3745375
TACGCAC1750.023.19957550-54
CATGGGG32950.023.1939525-29
CGATAGG452.6541722E-522.55514335-39
CCGCAAG4850.022.420351145
CGCGAAA1100.022.40714690-94
GCCCCAA6350.021.69066145
GAAACCG3650.021.44982785-89
Produced by FastQC (version 0.11.5)