FastQCFastQC Report
Sun 19 Mar 2023
SRR4895785_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895785_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709144
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG31700.4470178130252812No Hit
ATTGGCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC25080.3536658280969733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT711050.0143.811261
TGGCTCA209000.0143.413253
TTGGCTC713250.0143.367692
TGGCTCG226000.0143.345323
GGCTCGA65100.0142.988424
GCTCATG32800.0142.667955
GGCTCAT82400.0142.194144
TGGCTCT249950.0142.187443
GCTCGTA25750.0141.686615
GGCTCTA66100.0141.479694
GGCTCAG67550.0141.431494
GCTCGAT22200.0141.283635
GCTCTAG22150.0141.277025
GGCTCTG92500.0141.088764
GCTCGAA20350.0141.01795
GGCTCAA59500.0140.813644
GGCTCGT85650.0140.671024
GCTCATA21600.0140.53485
GCTCTGA21150.0140.45675
GCTCAGT23900.0140.28485
Produced by FastQC (version 0.11.5)