FastQCFastQC Report
Sun 19 Mar 2023
SRR4895785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709144
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG212893.0020701014180475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49740.701409022709069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA36780.5186534751757048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC12070.1702052051487427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11570.16315445100007897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC7610.10731247814266215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC7460.10519725189806302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT209900.072.053471
AGTGGTA214450.070.3168955
GTGGTAT214550.070.182746
GGTATCA215950.069.962758
AGCAGTG216400.069.922712
GTATCAA217100.069.5587549
TGGTATC217550.069.248257
CAGTGGT219550.068.65534
GCAGTGG223500.067.5068053
AGCGCAT907.4818214E-540.27633145
CGGTCTG3500.035.21302145
GGTCTGA5300.031.461132145
CGAGCCG1402.7462338E-531.070309145
CGCGAAA2550.027.2942990-94
CCGCAAG8300.027.077337145
CGCTGTT3750.027.065691145
CCGCGAA2700.026.31498190-94
TACGCAC3350.025.10357350-54
CGACCTA3400.024.30863245-49
TCTCGCA3750.023.20047470-74
Produced by FastQC (version 0.11.5)