FastQCFastQC Report
Sun 19 Mar 2023
SRR4895784_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895784_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907236
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC133491.4713922287034464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCAAG908250.0144.009121
ATCAAGT908550.0143.961582
TCAAGTG339500.0143.687453
TCAAGTT311100.0142.947713
CAAGTGT120800.0142.74364
TCAAGTA235650.0142.738043
CAAGTTG148350.0142.523654
CAAGTGG130300.0141.962814
CAAGTAG92000.0141.903494
CAAGTGA88050.0141.883424
CAAGTAT76950.0141.459054
CAAGTTA79700.0141.1914
TCAAGTC28100.0139.0453
CAAGTAA68100.0137.71864
CAAGTTT85000.0137.391074
AAGTGTG45200.0135.723025
AAGTATG35050.0135.53765
CAAGTCG10700.0135.417474
AAGTTAG37000.0133.849725
AAGTAGT31450.0133.631965
Produced by FastQC (version 0.11.5)