FastQCFastQC Report
Sun 19 Mar 2023
SRR4895784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907236
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG821949.059825668293586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA110701.2201896750128962No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97211.0714962810117765No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA21970.24216411165341764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC18170.2002786485545106No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA14800.1631328562799536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCTGACGTGGCCAAAAAA13680.15078766715606523No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG11620.12808133716034198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG10750.11849177060875009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA10650.11738952157983149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACTTGA9890.10901242896005009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9800.10802040483402335No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA9370.10328073400967333No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT9230.10173758536918728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT652050.050.288891
GGTATCA656700.049.883138
AGTGGTA656300.049.7754025
GTATCAA658550.049.756799
GTGGTAT656700.049.7395976
AGCAGTG659150.049.71422
TGGTATC660900.049.489337
CAGTGGT663650.049.202284
GCAGTGG666950.048.9697043
CGTGGGA9050.044.86317145
CCGCAAG7900.041.29866145
CGGTCTG1651.366061E-939.546597145
CGCAAGG4300.035.408145
CGCTGTT4300.033.721905145
CGCGGAA1050.007706994727.619846145
TGCGGCT3850.026.364397145
ATAGGCG4550.022.946983100-104
GCGATAG4750.022.894894105-109
CATGGGG47050.022.00464425-29
GGCGATA4950.021.676914105-109
Produced by FastQC (version 0.11.5)