FastQCFastQC Report
Sun 19 Mar 2023
SRR4895783_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895783_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119153
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG79340.7089289846875271No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC29250.26135836655041805No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGC12700.11347867539112166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC1122200.0143.805421
CATTCCG358050.0143.73423
TCATTCC1125900.0143.332842
ATTCCGA103000.0142.447374
CATTCCT398550.0142.261673
CATTCCA334900.0142.259783
ATTCCGT137550.0142.205524
ATTCCTG128650.0141.451254
ATTCCGG117600.0141.438454
ATTCCTA118850.0141.164154
ATTCCAT137550.0140.266114
ATTCCAG103250.0140.077414
TTCCGTA43100.0138.509755
TTCCGAT38200.0138.346395
TCCGTAT15300.0137.600086
TCCGTAG13350.0137.176486
TTCCGGA32350.0135.28255
TCCGAAT12000.0135.1856
TCCGAGT10950.0134.979256
TTCCGAG31650.0134.857535
Produced by FastQC (version 0.11.5)