FastQCFastQC Report
Sun 19 Mar 2023
SRR4895783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119153
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG366573.2754234675687774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98460.8797724707881763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA57750.5160152365226202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT331050.070.541331
GGTATCA334500.069.8104868
GTATCAA336900.069.374639
AGTGGTA335500.069.281375
GTGGTAT336000.069.0888756
TGGTATC336850.069.043687
AGCAGTG345800.067.4394762
CAGTGGT346700.067.100084
GCAGTGG352150.066.143993
CATGGGG53350.022.25879725-29
TACGCAC2400.021.1441250-54
CGGAGCC4151.0932126E-920.962345145
CGACCTA2350.020.3607845-49
GCGATAG1950.018.59073105-109
CATTCCG2359.1861375E-418.5150953
CGTGGGC7250.017.998701145
GCCCCAA8550.017.805735145
ATGGGAG26400.017.68496125-29
TCCGGCG2550.017.62665235-39
GAGGTAT3504.1632637E-516.5709746
Produced by FastQC (version 0.11.5)