FastQCFastQC Report
Sun 19 Mar 2023
SRR4895782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079532
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG132641.2286805763979205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75160.6962276245632367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG30660.2840119607385423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA22120.20490360637757843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18830.17442743707458416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11590.10736133806130806No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11450.10606447979309552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT206050.097.190361
GGTATCA207350.096.7773748
GTGGTAT207150.096.4158556
GTATCAA209200.095.9562159
TGGTATC210800.094.883987
AGTGGTA211250.094.578915
AGCAGTG211800.094.410512
CAGTGGT212650.093.964944
GCAGTGG219600.090.826023
TAGCGAC1600.077.033742
ATAGCGA2350.055.5362781
CGGTCTG2650.054.71621145
AGCGACG657.3356763E-444.6168253
CCGACGC1000.006075084728.999594145
AGCGACA2051.29006585E-524.7568953
TACGCAC3950.023.85931850-54
CATGGGG67300.023.76613425-29
CGACCTA3850.022.59603745-49
TGACGAC4100.022.2791440-44
AGGTATC2755.214026E-621.0896367
Produced by FastQC (version 0.11.5)