FastQCFastQC Report
Sun 19 Mar 2023
SRR4895781_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895781_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660773
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC661800.0143.986182
AGGCTAA661850.0143.97531
GCTAACT231300.0143.903243
GCTAACG218700.0143.85233
GCTAACA192350.0143.427733
CTAACGT79300.0143.214754
CTAACGG76050.0142.887574
CTAACGA62100.0142.589374
CTAACTG79550.0142.583394
TAACTAG23950.0142.107455
TAACGTG26650.0141.509265
TAACGTA23850.0141.493945
CTAACAG68600.0141.271824
TAACGGT24850.0141.02315
CTAACAT67300.0140.893544
CTAACTA69900.0140.604454
TAACGAG19800.0139.844835
TAACGGA20800.0139.361595
TAACGAT22100.0138.994545
TAACTGA22950.0138.55955
Produced by FastQC (version 0.11.5)