FastQCFastQC Report
Sun 19 Mar 2023
SRR4895781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660773
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG168032.542930779556671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54670.8273643142198606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52740.798156098993149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA27290.41300113654764947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC24600.372291240713528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA10100.15285128175636717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9900.14982452370178564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA9090.13756615358073043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT232400.093.880091
GGTATCA235350.092.442888
GTATCAA236900.092.021669
GTGGTAT235850.091.878046
CAGTGGT236950.091.618364
AGCAGTG238000.091.488352
TGGTATC236950.091.482117
AGTGGTA240400.090.206235
GCAGTGG241950.089.725023
AAATATA20400.041.9347145
CGGTCTG1051.8534466E-434.52251145
AAACCGC11600.026.3740185-89
AGGGGTA2301.1018019E-625.2183515
GCGCAGG12100.024.68502445-49
CGCAGAT12650.024.18486290-94
TCGATAG300.001495327724.16576140-144
GGGTATC2401.5972346E-624.1657587
GAAACCG12900.023.49136585-89
ATGGGAG35950.023.43589425-29
GCATTGA12750.023.42869830-34
Produced by FastQC (version 0.11.5)