FastQCFastQC Report
Sun 19 Mar 2023
SRR4895780_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895780_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015989
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT13600.13385971698512486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGC1017100.0144.043372
AGCCATG1017450.0143.993821
CCATGCT329450.0143.46233
CCATGCG356700.0143.45853
CATGCGA101150.0143.422294
CCATGCA297300.0143.357563
CATGCGT129400.0143.203414
CATGCAG99400.0143.045794
CATGCTG113950.0142.877614
CATGCAT108100.0142.7394
CATGCTA96850.0142.49414
CATGCGG122200.0142.318274
ATGCGAT37550.0141.902185
CATGCTT115800.0141.840334
ATGCGTA37850.0141.729955
CATGCAA89000.0141.45194
ATGCGTG39250.0141.267175
ATGCGAG33950.0140.808325
ATGCTGT40250.0140.802785
ATGCTAG29900.0140.589195
Produced by FastQC (version 0.11.5)