FastQCFastQC Report
Sun 19 Mar 2023
SRR4895780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015989
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG138411.3623178991111122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72140.7100470575960961No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21610.2126991532388638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11640.1145681695372686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185450.085.422111
GGTATCA186200.084.922298
AGTGGTA186100.084.8510365
GTGGTAT186050.084.678996
GTATCAA188300.084.1594249
TGGTATC188650.083.5888067
CAGTGGT190750.082.790734
AGCAGTG191850.082.391642
GCAGTGG197000.080.016913
CGGTCTG2300.047.281116145
CGACCTA3350.022.50675445-49
TACGCAC3450.021.85373950-54
TGCGGCT7000.021.749313145
CATGGGG52050.021.45175225-29
TGACGAC4000.019.57438340-44
CGGCGGT4200.018.64135235-39
ACATGGG169900.018.58740620-24
TACATGG178600.018.50193420-24
AGTACAT180250.018.42105720-24
GTACATG180750.018.40218520-24
Produced by FastQC (version 0.11.5)