FastQCFastQC Report
Sun 19 Mar 2023
SRR4895779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142777
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30360.26566863001267965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT1145150.0143.905291
GCACCTC1146300.0143.760932
CACCTCG382450.0143.13293
ACCTCGA111300.0143.041434
ACCTCGG130950.0142.115164
CACCTCA353500.0142.045443
ACCTCGT137100.0141.893484
ACCTCAG118250.0141.707524
ACCTCTG125950.0141.230644
CACCTCT386450.0141.128953
ACCTCAT130650.0141.116974
ACCTCTA121500.0141.002914
CCTCGAT38150.0139.29355
CCTCTAG39850.0138.960335
CCTCATG51100.0138.70455
CCTCGAG36800.0138.525445
CCTCGTA42600.0138.452765
CCTCAGT41600.0137.794435
CTCGTAT14450.0137.221686
CTCGAAT12250.0137.14436
Produced by FastQC (version 0.11.5)