FastQCFastQC Report
Sun 19 Mar 2023
SRR4895779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142777
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG179681.5723102582568604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93940.8220326450392335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24420.2136899850101988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG22120.1935635736456019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC15580.13633456046105233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT223000.086.8101651
AGTGGTA223500.086.2266855
GGTATCA224600.086.159478
GTGGTAT224950.085.667136
GTATCAA227050.085.222319
TGGTATC227500.084.806227
AGCAGTG229400.084.391962
CAGTGGT229850.083.816644
GCAGTGG238400.080.810633
CGGTCTG2900.052.498577145
CGCCGAA1157.144932E-637.82506145
GGCTATC5650.028.229322145
CCGACGC1050.00770992627.618298145
AGCGGTA1409.991945E-425.894425
CGATAGG3500.024.8571235-39
TACGCAC2050.024.04812850-54
CATGGGG68800.022.89106425-29
TGCGATA4000.021.0262730-34
ATGGGGG32500.020.838125-29
TCGTAAG4300.020.568848100-104
Produced by FastQC (version 0.11.5)