FastQCFastQC Report
Sun 19 Mar 2023
SRR4895778_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895778_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631151
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA28515217.481643330384493No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC425662.6095683354882535No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA378602.3210604045854737No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGTACTCTGCGTTGATA285861.752504826346549No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACTCTGCGTTGATA282861.7341129055495168No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGATA205001.2567812544638723No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA197501.2108014524712918No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGTTGATA152130.932654303617507No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTTGATA152110.9325316908121933No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATA142290.8723288034032409No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCATGTACTCTGCGTTGATA120680.7398456672619519No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA115250.7065562906193235No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCTGATA102190.6264901287495762No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATA93500.5732148648408394No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACGCTGCGTTGATA88490.5425003571097955No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGTTGATA87920.5390058921583594No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA82850.5079235460113748Illumina Single End PCR Primer 1 (100% over 21bp)
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGCGTTGATA67570.41424736275182367No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGGGTA65450.4012504053885876No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCAGGTACTCTGCGTTGATA65230.39990166453013853No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGCGGATA63960.39211575139272825No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGCGCGGCGGGTA63900.39174791297678757No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGTTGATA60150.36875801198049724No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACTCTGCGTTGATA59380.3640374189759256No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCATGTACTCTGCGTTGATAC58290.3573550210863372No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCTGATA55580.3407409859663514No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCATGTACTCTGCGTTGATA54560.3344877328953604No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGGACGCTGCGTTGATA49000.3004013730181939No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGTACTCTGCGTTGATA42480.2604295984859771No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCAGGTACTCTGCGTTGATA39050.23940150237470348No Hit
AGATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGTTGATA37940.23259649167980156No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGATGTACTCTGCGTTGATAC36900.22622062580349703No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGCGGGTA34750.21303974923229055No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGGACTCTGCGTTGATA34490.21144578276321443No Hit
AGATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA32950.20200459675407118No Hit
AGATGTACTCTGCGCTGATACCACTGCTTCCCATGTACTCTGCGTTGATA31690.19427999001931764No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAGA31640.19397345800603377No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTTGATA31610.19378953879806346No Hit
AGATGTACTCTGCCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30710.1882719625589538No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCATGTACTCTGCGTTGATAC30600.18759759212972926No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGTACGCTGCGTTGATA30330.18594231925799634No Hit
AGATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGTTGATA29440.18048604942154345No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGGTGAAAC28180.1727614426867899No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGGCGTGGATA28140.1725162170761628No Hit
AGATGTACTCTGCGTCGATACCACTGCTTCCCATGTACTCTGCGTTGATA27880.17092225060708666No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACTCTGCGTTGATA27670.1696348161512944No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCGCACGGACGCGGCGCGGATA27540.16883783291675633No Hit
AGATGTACTCTCCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA26420.1619715158191976No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCATGGACTCTGCGTTGATA26410.16191020941654083No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGTTGATA26210.16068408136340537No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGCGTGTA26070.1598257917262105No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCTGCGTGGATA26000.15939664690761307No Hit
AGATGTACTCTGCGTTGATACCACTGCTGCCCACGTACTCTGCGTTGATA24700.15142681456223245No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCGTGGATA24520.1503232993144105No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCATGGACGCTGCGTTGATA24020.1472579791815718No Hit
AGATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGTTGATA23820.1460318511284363No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGGACGCTGCGCTGATA22850.1400851300707292No Hit
AGATGTACTCTGCGTTGATAGCACTGCTTCCCATGTACTCTGCGTTGATA22680.13904292122556403No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGTACGCTGCGTTGATA22610.13861377640696662No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGCTGATA21940.13450624742896275No Hit
AGATGTACTCTGCGTTGATACCACTGCGGCCCATGTACTCTGCGTTGATA21880.1341384090130221No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGAACGGACGCGCGGCGGGTA21280.13046002485361563No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCGATA20910.128191687955315No Hit
AGATGTACTCTGCGTTGATACCACTGCAACCCATGTACTCTGCGTTGATA20180.12371632056137048No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCCACGTACTCTGCGTTGATA19990.12255149891089176No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGCTGATA19890.12193843488432402No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAGGTACTCTGCGTTGATAC19860.12175451567635369No Hit
AGATGTACTCTGCGTTGATACCACCGCTTCCCACGTACTCTGCGTTGATA19360.11868919554351498No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGGTGATAC17990.11029021837953691No Hit
AGATGTACTCTGCGTTGATACCACTGCGTCCCACGGACTCTGCGTTGATA17810.10918670313171497No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCGCTGATA17400.10667314062278724No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACTCTGCGTTGATA17160.10520178695902463No Hit
AGATGTACTCTGCGTTGATACCACTGCATCCATGTACTCTGCGTTGATAC17150.10514048055636786No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA400.0144.312218
AGATGTA1632300.0144.210541
GATGTAC1655450.0142.193862
TGTACGA24500.0141.661584
TGTACGT29600.0141.143194
GTACGTG9450.0139.730875
GTACTTG10400.0139.455555
TGTACAT22800.0138.932144
GTACGTT8950.0138.668725
GTACGAT9700.0138.361195
GTACGTA9950.0137.785525
TGTACTT34550.0137.629154
GTACTAA6150.0137.272585
TCTGCCT27600.0137.266089
TGTACGG32500.0136.985584
TGTACAA21300.0136.859454
GTACATG8350.0135.670765
TACGATT4000.0135.29276
TACGTGA3000.0134.691396
GTACATT6700.0134.619585
Produced by FastQC (version 0.11.5)