FastQCFastQC Report
Sun 19 Mar 2023
SRR4895778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631151
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG84853652.020689684768605No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1276967.828582393659447No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA190811.1697874690939098No Hit
AAGCAGTGGTATCAACGCAGAGTACATCTAGATCGGAAGAGCACACGTCT162430.9957998983539844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA143900.8821991342309817No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG116470.7140356717434498No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA91080.5583787153979001No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT89540.5489375293887567No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG82420.5052873706971335No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT79720.48873464197980443No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG78430.48082611603708053No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG78150.47910953676269086No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC77630.47592160382453863No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT76310.46782915867384445No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA70690.43337496038073725No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC70530.4323940579382289No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG67900.41627047403949724No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC67300.4125920898800908No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA66800.4095267697472521No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG65360.40069864776467656No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA64550.39573282914947794No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG63890.3916866065741308No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62260.38169366294107654No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT61130.37476603944086107No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60960.3737238305956959No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG58820.3606042604271462No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA58060.3559449738252314No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA57070.34987563996221077No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC53450.32768272220045846No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52920.3244334828596494No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT50830.3116204447043836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG46890.2874657220576145No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC42990.2635562250214726No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA32760.20083977510359247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTATCAACGCAG31880.19544481166979635No Hit
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG30690.18814934975364023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG28230.17306797470007373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCCACGCAG26670.16350417588561697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCCGTGGTATCAACGCAGA23620.14480572307530082No Hit
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC22250.13640674591132274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTATCAACGCAG21920.1343836346236492No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAACGCAG18550.11372337692831626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18160.11133242722470207No Hit
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT18140.11120981441938851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGG42100.083.523605145
TCCGGGT3850.077.209854145
TTCGGGT4550.066.92486145
TTCGGCG2250.064.44616145
TACGGGG3500.064.215996145
CTCGGCT14350.062.649754145
TCCGGCG2900.062.501667145
TACGGTA702.3350367E-762.144512145
TCCGGGG4200.058.69204145
CACCTAG7050.057.590187145
TCCGGCT8600.057.327106145
CCCGGGT6450.057.327106145
TTCGGCT20450.057.079758145
GATGTAC1154.0017767E-1156.7528232
CCCGGCC8850.056.526924145
TCCCGCC3600.056.390392145
CTCGGGT9300.056.13053145
CCCGGCG3500.055.93006145
CATCTCG6500.055.770718145
CACGGCC7200.055.38342145
Produced by FastQC (version 0.11.5)