FastQCFastQC Report
Sun 19 Mar 2023
SRR4895777_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895777_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100491
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1101950.0144.008852
ACAGATT1102900.0143.88481
AGATTCG371550.0143.739413
GATTCGA110900.0143.294924
AGATTCA323150.0143.245713
GATTCAG111900.0143.17424
GATTCGT135700.0142.87684
GATTCGG123100.0142.740844
GATTCTG121950.0142.313164
ATTCGAG38400.0142.139715
AGATTCT383900.0142.007693
GATTCAT115650.0141.461944
GATTCAA96350.0140.388614
GATTCTA114650.0140.243124
ATTCGAA30400.0140.17315
ATTCGAT40250.0139.547595
ATTCGTA40650.0139.416065
ATTCGTG43900.0138.949375
ATTCGGA33700.0138.642335
ATTCGGT43850.0138.614525
Produced by FastQC (version 0.11.5)