FastQCFastQC Report
Sun 19 Mar 2023
SRR4895777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100491
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG203871.8525367313317418No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97680.8876038059375315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33120.3009565730205881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14010.12730681123244078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11550.10495315272910002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT232600.081.01621
GGTATCA235450.079.7783058
AGTGGTA235650.079.5024645
GTGGTAT236000.079.2544866
GTATCAA237350.079.231319
CAGTGGT238900.078.3905644
AGCAGTG240150.078.284452
TGGTATC239350.078.175517
GCAGTGG246350.076.019923
CGGTCTG3400.040.5145145
CGGAGCC4550.031.867973145
TACGCAC3250.024.98426250-54
CGACCTA3050.024.72006645-49
CCGCAAG10050.023.084463145
CCGCGAA2750.021.6170990-94
CATGGGG53300.021.4108125-29
GTCGGTA1250.020.87894690-94
CGCGAAA2850.020.85859790-94
TCGTCGG1400.019.67757890-94
GTTCTCG3100.019.176973110-114
Produced by FastQC (version 0.11.5)