FastQCFastQC Report
Sun 19 Mar 2023
SRR4895776_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895776_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938001
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACGACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG46260.4931764465069866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC941550.0143.659011
CACGACC942500.0143.514222
ACGACCT305750.0143.47173
ACGACCG296350.0143.156543
ACGACCA286750.0143.020943
CGACCAT105350.0142.62864
CGACCGA90200.0142.604024
CGACCGT102350.0142.582464
GACCGAT30250.0142.534265
CGACCTG101700.0142.50124
CGACCTT114500.0141.87284
CGACCTA89600.0141.78864
GACCATG43100.0141.526115
CGACCAG95800.0141.342764
GACCGTA32350.0140.413775
CGACCGG101700.0140.161644
GACCGGT29450.0140.040665
GACCTGT33650.0139.488825
GACCGTG30250.0139.435685
GACCGTT31350.0139.142995
Produced by FastQC (version 0.11.5)