FastQCFastQC Report
Sun 19 Mar 2023
SRR4895776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938001
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG193652.064496732945914No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64620.6889118455097596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33800.36034076722732705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT313250.098.238461
AGCAGTG315800.097.297072
GGTATCA316500.097.048628
AGTGGTA317400.096.5324865
TGGTATC317200.096.468787
GTGGTAT317050.096.468696
CAGTGGT319350.095.988464
GTATCAA323850.094.913199
GCAGTGG324750.094.4373
GCCCCAA6200.026.895962145
TGCGGCT4700.024.681585145
CATGGGG57300.024.47184425-29
CGACCTA2300.023.9546845-49
CGGTCTG1851.8301787E-423.514215145
AGCGCAT1550.001808092323.387793145
TACGCAC2550.022.1759550-54
ATGGGAG46600.021.8135825-29
ATGGGAT49100.021.73657625-29
CCGACCC1700.00309211921.324163145
ATACGAG1700.00309211921.324163145
Produced by FastQC (version 0.11.5)