FastQCFastQC Report
Sun 19 Mar 2023
SRR4895775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633290
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT55250.8724281135025028No Hit
AATGTTGCGTTGATACCACTGCTTCCCATGTACTCGGCGTTGATACCACT6800.10737576781569265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC634550.0143.903722
AATGTTG637000.0143.350221
TGTTGCG233550.0143.211263
TGTTGCA174250.0143.164723
GTTGCGA67100.0142.802264
TTGCGGA17800.0142.578925
TTGCGAT23800.0142.381485
GTTGCGT94500.0141.681374
GTTGCAA51750.0141.551994
GTTGCAG61550.0141.504914
GTTGCAT61050.0141.482854
GTTGCTA60950.0141.47844
TGTTGCT210600.0141.391833
TGCGTAA6700.0140.97086
TGCGTGA8500.0140.806216
TTGCGTA21900.0140.248475
GTTGCGG72000.0140.093464
TGCGATA6800.0139.9586
GTTGCTG79000.0139.818394
TTGCGAA19850.0139.477235
Produced by FastQC (version 0.11.5)