FastQCFastQC Report
Sun 19 Mar 2023
SRR4895775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633290
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG237623.7521514629948363No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55630.8784285240569092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32700.5163511187607573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA9090.14353613668303622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7610.12016611662903252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT223550.066.783421
AGTGGTA226600.065.783335
GTGGTAT226750.065.702656
GGTATCA227650.065.6658258
GTATCAA228200.065.634639
TGGTATC227750.065.4467
CAGTGGT230150.064.7371444
AGCAGTG231550.064.439672
GCAGTGG238600.062.5052573
CGGTCTG1551.4551915E-1146.775772145
CGCAAGG2951.4253601E-824.577103145
CCGCAAG4750.024.421877145
ATGGGGG19500.021.49080825-29
CGCGAAA1350.021.48186790-94
CATGGGG45250.020.8297625-29
TTAGCGC708.755342E-820.7133540-44
CGACCTA1200.020.5407445-49
TACGCAC1300.020.07633250-54
ATGGGGC13650.019.86545425-29
ACTATCG1005.4387783E-1018.850637140-144
Produced by FastQC (version 0.11.5)