FastQCFastQC Report
Sun 19 Mar 2023
SRR4895774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964569
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC965500.0144.067372
AATCCGT965550.0144.059921
TCCGTCA289850.0143.537613
TCCGTCG326100.0143.324833
CCGTCAG95900.0143.081454
CCGTCTA97850.0142.809194
TCCGTCT327200.0142.512443
CCGTCAT106750.0142.45314
CCGTCGA95400.0142.319734
CCGTCAA86750.0142.297564
CGTCAAT32250.0141.973465
CGTCGTA35300.0141.758135
CCGTCGT120200.0141.749794
CGTCAGT34100.0141.671885
CGTCATG35100.0141.538735
CGTCGAT34500.0141.283285
CGTCTAG33500.0140.98075
CCGTCTG100350.0140.975884
CGTCGGT37450.0140.743625
CGTCTAA28750.0140.698095
Produced by FastQC (version 0.11.5)