FastQCFastQC Report
Sun 19 Mar 2023
SRR4895774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964569
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG128981.3371775373249608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69010.7154490762195344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21600.22393421310450573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15990.16577352164541884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT178950.088.970731
GGTATCA179250.088.619598
GTATCAA180650.087.928269
AGTGGTA180400.087.8984765
GTGGTAT180550.087.7004246
TGGTATC181600.087.2731867
CAGTGGT184000.086.2969364
AGCAGTG184400.086.267022
GCAGTGG192100.082.658173
TCCGTCG907.479089E-540.280663
ATCCGTC3300.030.7597752
AATCCGT3503.6379788E-1226.9291021
CATGGGG52400.022.49851225-29
TACGTCG350.003661412520.71362100-104
CGACCTA2250.019.97713545-49
TACGCAC2200.019.77209350-54
ACATGGG162300.019.1564220-24
TACATGG172200.019.04879620-24
AGAGTAC177750.018.8595915-19
CAGAGTA178000.018.79237215-19
Produced by FastQC (version 0.11.5)