FastQCFastQC Report
Sun 19 Mar 2023
SRR4895773_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895773_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791378
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCTG792300.0144.017172
CAGATCT792700.0143.944491
GATCTGG295800.0143.531983
GATCTGA210350.0143.436043
GATCTGT250000.0143.267233
ATCTGGA76250.0143.154
ATCTGGT100050.0142.676824
ATCTGAG82650.0142.619934
ATCTGTG106550.0142.498474
GATCTGC39850.0142.019063
ATCTGTA70150.0141.929064
ATCTGAT71700.0141.87744
ATCTGGG118300.0141.630464
ATCTGAA55350.0139.891714
TCTGAGT25550.0138.837555
TCTGAGG32900.0138.501345
TCTGGTA29000.0137.735085
ATCTGCG15600.0137.724
TCTGGAT24350.0137.388955
TCTGAGA19050.0137.386615
Produced by FastQC (version 0.11.5)