FastQCFastQC Report
Sun 19 Mar 2023
SRR4895773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791378
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG249853.1571511970259474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67650.8548380167252564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA40220.5082274210301525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14340.18120291441005434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9720.12282373278003686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT244800.075.0487751
AGTGGTA243700.074.996035
GGTATCA247000.074.258228
GTATCAA247650.073.9754949
GTGGTAT249050.073.3849956
AGCAGTG251700.072.900392
CAGTGGT250850.072.829514
TGGTATC251500.072.785427
GCAGTGG256700.071.2262653
CGGTCTG2451.9717845E-929.590675145
CGCTGTT4400.026.362602145
CCGCAAG9100.025.493505145
CGATCGA1600.002175247322.6553616
CGACCTA2400.022.3535745-49
CATGGGG44650.021.98569125-29
TACGCAC2600.021.19147750-54
CGGCGGT2750.021.0903535-39
ATAGGCG3250.020.97053100-104
GCGATAG3250.020.969204105-109
CACGGGA4752.728484E-1019.841328145
Produced by FastQC (version 0.11.5)