FastQCFastQC Report
Sun 19 Mar 2023
SRR4895772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213879
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG119080.9809873966021325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99120.8165558511186042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18990.15644063370401828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT190500.092.1435851
AGTGGTA191000.091.678385
GGTATCA191750.091.50518
GTGGTAT192150.091.088216
GTATCAA193050.090.765019
TGGTATC193900.090.34097
CAGTGGT195050.089.856544
AGCAGTG196000.089.457992
GCAGTGG203350.086.153263
CGGTCTG2507.094059E-1131.898062145
CGATAGG3250.024.98371535-39
CGTATGC5150.023.930584145
CATGGGG60750.022.58214425-29
TCGTAAG4200.021.405224100-104
CGACCTA3600.021.346145-49
TACGCAC3400.021.32223350-54
GGTCGCG4550.021.03168950-54
TACATGG181700.020.03020720-24
ACATGGG176550.019.8014120-24
AGAGTAC186200.019.79417815-19
Produced by FastQC (version 0.11.5)