FastQCFastQC Report
Sun 19 Mar 2023
SRR4895771_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895771_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859975
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG250650.0143.74683
AGAGATC863050.0143.660342
GAGATCA254750.0143.414173
AAGAGAT864900.0143.353041
AGATCAG81800.0143.120674
AGATCGA81100.0143.111544
AGATCTG101950.0143.046844
GATCGAG24800.0142.724815
AGATCGT104250.0142.449464
GAGATCT338150.0142.408773
GATCGAT30150.0142.26545
GATCATG29200.0141.70945
AGATCAT95150.0141.678014
GATCTGA25950.0141.678015
GATCTAG33450.0141.592065
AGATCAA78250.0141.506534
AGATCTA104700.0141.492944
GATCGAA22850.0141.33885
GATCGTA30350.0141.327915
GATCAGT30750.0141.130525
Produced by FastQC (version 0.11.5)