FastQCFastQC Report
Sun 19 Mar 2023
SRR4895771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859975
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG127381.4812058490072386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82260.9565394342858804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36830.4282682636123143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19720.22930899154045176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19350.22500654088781652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT188050.095.9568561
GGTATCA190550.094.094638
GTATCAA191700.093.713819
GTGGTAT191250.093.409056
AGCAGTG193900.092.842872
TGGTATC193500.092.3978357
CAGTGGT194500.091.960054
AGTGGTA196100.091.1357965
GCAGTGG202800.088.2321553
CGGTCTG2451.8189894E-1235.50703145
CGCGAAA1350.025.77547390-94
GCCCCAA6550.025.45574145
CATGGGG51400.023.4142125-29
CGACCTA2350.022.82933845-49
AAATATA26150.022.732191145
CGTTCGA1001.8189894E-1221.74881485-89
GAAACCG9150.021.55073785-89
AAACCGC9150.021.39227785-89
CGCAGAT9450.021.1727190-94
AGGTATC2755.208054E-621.0914767
Produced by FastQC (version 0.11.5)