FastQCFastQC Report
Sun 19 Mar 2023
SRR4895769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903696
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40760.2141098158529513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1905400.0144.076572
TTCACGC1907450.0143.921741
CACGCAT679000.0143.709923
ACGCAGT227250.0143.701354
CACGCAG630350.0143.568453
ACGCATG232950.0143.435214
ACGCATA203150.0143.428164
ACGCATT237500.0143.298224
ACGCAAT206150.0143.124734
CACGCAA563900.0142.840843
CGCAGTA72500.0142.717215
CGCATAG66250.0142.68535
CGCATTA67850.0142.083625
ACGCAGA181700.0142.066124
ACGCAAG194700.0142.061074
CGCATTG79950.0142.044545
ACGCAGG218800.0141.803344
CGCATAT70600.0141.757895
CGCAAGT68450.0141.680885
CGCATGA61600.0141.165655
Produced by FastQC (version 0.11.5)