FastQCFastQC Report
Sun 19 Mar 2023
SRR4895769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903696
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG231351.2152675637286625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171540.9010892495440449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG142970.7510127667442701No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC73340.3852505862280532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35520.18658441263731182No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG20150.10584673183113269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT336200.095.2140051
GGTATCA340550.093.9289868
GTGGTAT340650.093.2629246
GTATCAA344500.092.886779
AGCAGTG346050.092.338632
TGGTATC345050.092.199727
CAGTGGT346500.091.8605654
GCAGTGG358550.088.7126853
AGTGGTA364600.087.260525
TTCACGC5100.048.336651
TCACGCA5550.044.4186332
CGGTCTG4200.039.70198145
AAATATA57100.037.07493145
AGGTATC5300.030.0948247
GAGGTAT5350.028.4584036
CGGAGCC8300.027.951525145
CCGCAAG13050.027.221945145
GAAACCG30550.024.63184485-89
AAACCGC30000.024.55179485-89
CATGGGG98750.024.41925-29
Produced by FastQC (version 0.11.5)