FastQCFastQC Report
Sun 19 Mar 2023
SRR4895768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545677
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG133570.8641520835206837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89080.5763170442466311No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG36420.23562490740303438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT34930.22598511849500252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22940.14841393124177948No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21700.1403915565800617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC20130.13023419511320927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT279000.0102.5740661
GGTATCA278900.0102.389618
GTGGTAT279050.0102.04886
GTATCAA281600.0101.404619
TGGTATC282050.0101.066187
AGCAGTG286500.099.759122
CAGTGGT287050.099.290134
AGTGGTA289100.098.510835
GCAGTGG296650.095.979213
CGGTCTG3400.038.380623145
CGCTGTT7200.034.23457145
CGGAGCC6350.030.825384145
CCGCAAG14800.025.961668145
CATGGGG66400.022.9736425-29
CGACCTA3300.022.4080845-49
TACGCAC3250.022.30726250-54
TACATGG270600.021.70290420-24
AGAGTAC276950.021.65403615-19
CAGAGTA277950.021.58656315-19
GTACATG275050.021.58374420-24
Produced by FastQC (version 0.11.5)